PURIFICATION AND PROTEOLYTIC ACTION OF MILK- CLOTTING ENZYME PRODUCED BY PENICILLIUM CITRINUM
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چکیده
منابع مشابه
Purification and Characterization of Milk Clotting Enzyme Produced by Rhizomucor Rmiehei
Milk clotting enzyme (M CE) produced by: Rhizomucor miehei was purified and characterized.The enzyme was purified 220.29-fold with specific activity about 14444.2 U/mg protein byultrafiltration, ammonium sulfate fractionation, Sephacryl S-300 chromatography. The maximumenzyme activity was at 65°C.The milk clotting activity was decreased steadily as pH is increased and indicated maximumactivity ...
متن کاملPurification of endoglucanase produced by Penicillium citrinum isolated from Amazon
Background The cellulolytic enzyme complex has many important biotechnological applications such as beverage, textile, food, paper and cellulose industries, as well as in degradation of lignocellulosic for ethanol biofuel production [1]. According to the enzymatic activity, cellulolytic complex is subdivided in three classes: endoglucanases, exoglucanases and b-D-glycosidases. Endoglucanases (e...
متن کاملpurification and characterization of milk clotting enzyme produced by rhizomucor rmiehei
milk clotting enzyme (m ce) produced by: rhizomucor miehei was purified and characterized.the enzyme was purified 220.29-fold with specific activity about 14444.2 u/mg protein byultrafiltration, ammonium sulfate fractionation, sephacryl s-300 chromatography. the maximumenzyme activity was at 65°c.the milk clotting activity was decreased steadily as ph is increased and indicated maximumactivity ...
متن کاملEndo and exoglucanases produced by Penicillium citrinum isolated from Amazon
Background Cellulolytic enzymes (glucohidrolases EC 3.2.1.-) are biocatalizators highly specific. They act in synergy to hydrolyze b-1,4 bonds between monosaccharide units of D-glucose in the cellulose chain releasing its constituents. Cellulases are categorized according to the place they act in the cellulosic fiber. Endoglucanases start hydrolysis, exoglucanases act in the reduced terminal pr...
متن کاملExtraction of milk-clotting enzyme produced by solid state fermentation of Aspergillus oryzae.
Studies on the extraction of milk-clotting enzyme after solid-state fermentation (SSF) of wheat bran by a local strain of Aspergillus oryzae LS1 were done. The extraction of the enzyme was found to be depended on different parameters like nature of extractant, soaking time, temperature etc. From different inorganic and organic extractants, calcium chloride (0.05%) and glycerol (40%) were found ...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1972
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.18.341